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KMID : 0385520220350030124
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Analytical Science & Technology
2022 Volume.35 No. 3 p.124 ~ p.128
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False positive and false negative reactions of acidic hydrogen peroxide for enhancing blood
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Lee Won-Young
Hong Sung-Wook
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Abstract
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Blood-sensitive reagents may exhibit false positives or negatives under the influence of substances other than blood. Since these reactions lead to the misinterpretation of blood evidence, it is essential to investigate the possibility of false-positive and -negative reactions of blood-sensitive reagents. Acidic hydrogen peroxide (AHP) is a recently discovered blood-sensitive reagent, and it is not yet known whether it causes false-positive or -negative reactions. To confirm this, 20 μL of blood was placed on metal surfaces, plastic surfaces, paper surfaces, paint surfaces, foods, vegetable oils, detergents, and petroleum hydrocarbons, and then AHP was applied. The blood was observed through an orange filter under a 505-nm light source, and no false-positive or false-negative reactions were observed with any of the substances/materials. However, it was confirmed that polyethylene terephthalate surfaces, polyvinylchloride surfaces, some paint surfaces, and foods exhibit their own photoluminescence under the conditions of blood observation, which interferes with blood observation.
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KEYWORD
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acidic hydrogen peroxide, false positive, false negative, blood enhancement reagent, forensic
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